You are currently viewing South Africa: Testing Stool Can Help Detect TB in Kids

South Africa: Testing Stool Can Help Detect TB in Kids


Most tuberculosis (TB) testing today relies on sputum samples coughed up from the lungs. This makes intuitive sense since pulmonary (or lung) TB is the most common form of TB and sputum provides testable samples straight from the scene of infection.

A major snag, however, is that children and some people living with HIV find it difficult or impossible to cough up the sputum needed for testing. The resulting delays or uncertainties in diagnosis often have knock-on effects on treatment.

“Confirming TB in children is challenging as the disease is frequently paucibacillary (which means the amount of TB in test samples is low and hard to detect),” says Dr Pedro da Silva, Operations Manager and Mycobacteriologist at Wits Diagnostic Innovation Hub and the National Priority Programme of the National Health Laboratory Service (NHLS). “Children find it difficult to expectorate (cough up specimen) and when it is possible, the specimen volume may be suboptimal. To get a good respiratory specimen from a child, more-invasive methods are frequently used, such as sputum induction. Often, skill sets to conduct the procedure or specialised equipment are unavailable. Caregivers may also refuse the invasive procedures.”

The race is thus on to find TB tests that do not rely on sputum.

Blood, urine, and saliva are all candidates, as increasingly is stool. While all these have pros and cons and while the evidence base is still evolving, there seems to be a compelling case for the wider use of stool samples.

Already WHO recommended

Dr Anna Mandalakas, a paediatric specialist, Chief of the Section on Global and Immigrant Health, and director of the Global Tuberculosis Programme of Texas Children’s Hospital tells Spotlight that over the past decade, an increasing amount of research has been completed to study the use of stool samples for the laboratory confirmation of TB disease. Their Baylor Global TB team recently demonstrated that, in addition to just testing for TB, testing of stool samples using gene sequencing technology can be used to determine whether someone’s TB is resistant to medicines used to treat TB. As Spotlight recently reported, such drug-susceptibility testing is becoming increasingly common.

The research is sufficiently advanced that, in 2021, the World Health Organization (WHO) recommended the use of stool as a new sample type when diagnosing TB in children. In 2022, the WHO published guidelines on how to do these tests.

According to Petra de Haas, technical officer of laboratory services at the KNCV Tuberculosis Foundation, at least three countries (Vietnam, Zambia, and Ukraine) have already adopted stool testing in their guidelines for routine testing, six countries are doing pilot implementations, and many other countries are preparing for the implementation of stool testing.

Stool-based TB diagnosis is not yet being implemented in South Africa. But, according to da Silva, the addition of stool as an additional specimen type for TB diagnosis in children in the country is under discussion and implementation is being planned with likely introduction in 2023.

Many people with TB in their lungs swallow their sputum, which contains TB organisms. These don’t usually survive in our intestines, but their DNA can sometimes be detected in their stool. This could help us diagnose TB when we’re struggling using other methods. – Dr Gary Reubenson

Why stool testing works

While sputum samples have the advantage of coming straight from the lung, the TB bacteria present in stool samples have taken something of a detour.

“Many people with TB in their lungs swallow their sputum, which contains TB organisms,” explains Dr Gary Reubenson, a specialist in paediatric infectious diseases at the department of paediatrics and child care Empilweni Service and Research Unit at Rahima Moosa Mother and Child Hospital. “These don’t usually survive in our intestines, but their DNA can sometimes be detected in their stool. This could help us diagnose TB when we’re struggling using other methods,” he says.

“For a long time,” says De Haas, “stool was considered a difficult sample for diagnosing bacterial diseases, as it contains a lot of other bacteria and organic material which interfere with diagnostic tests.”

This, however, changed with the introduction of rapid molecular tests that can detect genetic material from the TB bacterium.

The good news here is that the same molecular testing platform (GeneXpert) widely used in South Africa to test sputum samples for TB can also be used to test stool samples. But as Reubenson points out, there are some differences in how samples are processed prior to being tested.

Eligibility and sample collection

Da Silva says the use of stool as a diagnostic test for TB using the currently available molecular test is only recommended for children under the age of 10. “In other age groups, the current sputum and extra-pulmonary specimen types can be tested depending on signs and symptoms and presenting features of the disease. In individuals with advanced HIV infection where expectoration of sputum may be difficult, alternate tests can be performed such as detection of lipoarabinomannan in urine specimens (using the point of care urine-LAM assay),” he says.

The WHO’s practical manual for processing stool samples for diagnosis of childhood TB suggests that stool collection should usually be done by the caregivers or the patients themselves, depending on the age of the child. “Ideally, the collection takes place at the healthcare facility. However, to obtain a specimen on demand is often challenging, therefore, stool is collected at home and the patient or caregiver needs to return to the facility for specimen submission,” reads the manual.

Stool samples are put into the same containers that are often used when collecting sputum and then taken to the Xpert machine.

Stool samples do not have any special storage requirements, according to Reubenson. “They are put into the same containers that are often used when collecting sputum and then taken to the Xpert machine. These machines can be found in lots of places in South Africa; many laboratories have them, but some clinics and hospitals too,” he says.

“The test concludes whether TB bacilli are present in the stool sample and quantifies the number of bacteria. It also reports if there are mutations in the DNA that lead to resistance to one of the first-line anti-TB drugs called rifampicin,” says De Haas.

It takes two hours to run the test, but since samples often have to be transported from health facilities to labs, the time from handing over a sample to getting a result can be much longer.

The test concludes whether TB bacilli are present in the stool sample and quantifies the number of bacteria. It also reports if there are mutations in the DNA that lead to resistance to one of the first-line anti-TB drugs called rifampicin. – Petra De Haas

Stool compared to other methods

“Current evidence suggests that the performance of GeneXpert is similar for sputum and stool specimens. There is a need for more evidence examining the performance of GeneXpert on various specimens provided by different populations, particularly children and people living with HIV,” says Mandalakas.

Morten Ruhwald, director of the TB programme at the Foundation for Innovative Diagnostics (FIND), concurs that stool-based testing seems comparable to sputum-based testing and points out that the two methods are complementary.